Fig 1: LPM induction from hPSCs.a Schematic diagram of NK cell regeneration. b Cell images at specified time points. c Real-time quantitative PCR analysis of TBXT and APLNR gene expression levels at selected time points. β-ACTIN gene was used as internal control. The relative gene expression levels were calculated as 2–ΔCt. d Dynamic analysis of BRACHYURY+ primitive streak (PS) cells at selected time points. e Dynamic analysis of the APLNR+ LPM cells. f Statistics of iLPM cell yields per hPSC input. n = 6 each group. Data were collected from two independent experiments. Two-tailed independent t-test. ESC: human ESC line hPSC-2; iPSC: human iPSC line hPSC-6.